The indicator dilution method helps in the determination of rate of blood flow and not the velocity of blood. Any substance having no toxic side effects can be used as an indicator if it readily mixes with blood and its concentration can be easily determined after mixing. This principle is used in the indicator dilution method. Here the substance used should be stable but should not be retained in the body. Among various types of indicators indo cyanine dye and cardio green are widely used. Radioisotopes also been employed for this purpose. The most frequently used indicator is isotonic saline. There are two types of measurements involved in this method, they are, open circulation method and closed circulation method.
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